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Abstract

A B S T R A C T
Introduction To explore the effect of ethynil estradiol and desogestrel on
proliferation and apoptosis hydatidiform mole trophoblast cell. Methods: From April
2008 until March 2009, we collected 15 samples of hydatidiform mole tissue.
Trophoblast cells was isolated and culture at RPMI 20% FBS medium. Only 7
samples (46%)shown good growth. Cell was identified using cytology and β HCG test.
Trophoblast cells good quality than devided into three groups observation. First
group get ethynil estradiol 10 nmol/mL, second group get desogestrel 100 nmol/mL,
Third group get DMSO 1%. Cells incubated and observe at 24, 48, 72, 96 hours. Cell
cycle, apoptosis and β HCG was evaluated at each time observation. Cell cycle
evaluation using BD cycle test plus DNA reagent, apoptosis evaluation using FITC-
Annexin V. Analyze using FACSCalibur flowcytometer. β HCG evaluation using
Abbott AxSym total β-HCG reagent pack. Results: The group of cell that get ethynil
estradiol in concentration 10 nmol/mL had cell proliferation index, amount cells and
β HCG level higher than control after 72 hours observation. The group of cell that
get desogestrel in concentration 100 nmol/mL have cell proliferation index, amount
cells and β HCG level lower than control after 48 hours observation. There are no
differences of apoptosis between the two group and control. Conclusion: Ethynil
estradiol will increase proliferation of hydatidiform mole trophoblast cell, while
desogestrel will decrease proliferation of hydatidiform mole trophoblast cell. There
are no effect of ethynil estradiol and desogestrel on apoptosis of hydatidiform mole
trophoblast cell.

Keywords

Hydatidiform mole Trophoblas Proliferation Apoptosis Ethynil estradiol Desogestrel

Article Details

How to Cite
Sastradinata, I., Andrijono, Mohamad Farid Aziz, Wan Lelly H, Sri Hartini, & Bambang Sutrisna. (2021). The Effect of Ethynil Estradiol and Desogestrel on Proliferation and Apoptosis Hydatidiform Mole Trophoblast Cell. Bioscientia Medicina : Journal of Biomedicine and Translational Research, 5(2), 312-319. https://doi.org/10.32539/bsm.v5i2.223