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Abstract

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

Keywords

ELISA Antigen Antibody Guideline

Article Details

How to Cite
Hidayat, R., & Patricia Wulandari. (2021). Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline. Bioscientia Medicina : Journal of Biomedicine and Translational Research, 5(5), 447-453. https://doi.org/10.32539/bsm.v5i5.228

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