The Effect of Active Gambir (Uncaria gambir) Fraction on TNF-α Protein Expression and Lesion Size in White Rats Gastritis Model

Lunsi Oktafitria#, Ellya Aidia, Rahmad Dhani, Ni Wayan Lisa Suasti Program Studi Sarjana Farmasi Sekolah Tinggi Ilmu Farmasi Bhakti Pertiwi

  • Ni Wayan Lisa Suasti STIFI Bhakti Pertiwi

Abstract

Background: Gambir (Uncaria gambir) is empirically used to treat abdominal pain and vomittus caused by gastritis because of its anti-inflammatory effects, especially flavonoid. This study aims to determine the effect of active gambir fraction on TNF-α protein expression and wound size in white rats gastritis model.


Methods: The research method used experimental study design in vivo with post test with control group design. Rats were divided randomly in 11 groups and were induced to be gastritis for 1 day. Group 1 (negative control) was given aquadest of 5 mL, group 2 (positive control) was administered ranitidine 10 mg/kgBW, groups 3, 4, and 5 were given n-hexane fraction, groups 6, 7 and 8 were given a water fraction, and groups of 9, 10, and 11 were given ethyl fractions with each group receives dose of 20, 40, and 80 mg/kgBW and all groups were treated for 3 days. Rats were dissected on 5th day for examination of gastric mucosal lesion size and performed ELISA expression of TNF-α expression of gastric mucosal tissue. The results of this study were assayed by SPSS 18.


Results: The result of the research using Kruskal-Wallis test showed that there were significant differences (p <0.05) of the lesions size between the sample groups where  control positive, ethyl fraction 20, 40, 80 mg/kgBW, and water fraction 20, 40 mg/kgBB had the gastric ​​mucosal lesion size differed significantly with the negative control group, while the TNF-α protein expression test  using Kruskal-Wallis showed that there was a significant difference (p <0.05) TNF-α levels of all groups against the negative control.


Conclusion: Active gambir fraction had a potention to reduce size of mucose gaster lesion and reduce expression of TNF-α protein.


 


Keywords: Gambir – TNF-α – antiinflamatory – gastiritis

Published
2018-12-06
How to Cite
SUASTI, Ni Wayan Lisa. The Effect of Active Gambir (Uncaria gambir) Fraction on TNF-α Protein Expression and Lesion Size in White Rats Gastritis Model. BioScientia Medicina, [S.l.], v. 2, n. 3, p. 1-7, dec. 2018. ISSN 2598-0580. Available at: <http://bioscmed.com/index.php/bsm/article/view/53>. Date accessed: 12 dec. 2018. doi: https://doi.org/10.32539/bsm.v2i3.53.