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Abstract

A B S T R A C T
The use of synthetic pyrethroids in insecticide-treated bed nets (LLINs) and spray
form (IRS) has been carried out since 2012 in Muara Emil Village and since 2016 in
all villages in Tanjung Agung District, Muara Enim Regency. Biochemical resistance
can occur to the enzymes that play a role in detoxifying the insecticide. This study
aims to identify whether there is an increase in the levels of the enzyme Cytochrome
P450 monooxygenase (CYPs) through the enzyme biochemical test on the malaria
vector mosquito Anopheles vagus originating from Pagar Dewa Village and Muara
Emil Village, Muara Enim Regency. The sample of female Anopheles mosquitoes was
not full. Blood was taken at night using the resting collection method. Mosquitoes
that have been identified as Anopheles vagus species are used to check CYPs enzyme
levels using the ELISA method. The results of the biochemical test showed that
enzyme levels increased from the Muara emil and Pagar Dewa village mosquitoes
with a percentage of 71% and 61.53% (with cut off point OD> 0.165). The value of
CYPs enzyme levels for mosquitoes from Muara Emil village was higher than that of
the mosquitoes from Pagar Dewa Village (mean 0.005027 ± 0.007). The increased
activity of the CYPs enzyme plays a role in the detoxification of synthetic pyrethroid
insecticides that can cause resistance. The high levels of enzymes from the villages
of Muara Emil and Desa Farah Dewa are in line with intensive insecticide exposure
in eradicating malaria vectors in the highest malaria transmission areas in Muara
Enim Regency.

Keywords

Pyrethroid synthetics Resistance Biochemical assay Enzyme-linked Immunosorbent Assay Cytochrome P450 monooxygenase CYPs

Article Details

How to Cite
Dalilah, Muhaimin Ramdja, Dwi Handayani, Lasbudi P Ambarita, R. Irpan Pahlepi, & Ahmad Ghiffari. (2021). Activity of Cytochrome P450 Monooxygenase (CYPs) Metabolic Enzymes as Markers of Insecticide Resistance in Anopheles vagus Muara Enim Mosquitoes, Indonesia. Bioscientia Medicina : Journal of Biomedicine and Translational Research, 5(2), 305-311. https://doi.org/10.32539/bsm.v5i2.222