The The Effect of Active Gambir (Uncaria gambir) Fraction on TNF-α Protein Expression and Lesion Size in White Rats Gastritis Model
DOI:
https://doi.org/10.32539/bsm.v2i3.53Keywords:
Gambir, TNF-α, Antiinflamatory, GastiritisAbstract
Background: Gambir (Uncaria gambir) is empirically used to treat abdominal pain and vomittus caused by gastritis because of its anti-inflammatory effects, especially flavonoid. This study aims to determine the effect of active gambir fraction on TNF-α protein expression and wound size in white rats gastritis model.
Methods: The research method used experimental study design in vivo with post test with control group design. Rats were divided randomly in 11 groups and were induced to be gastritis for 1 day. Group 1 (negative control) was given aquadest of 5 mL, group 2 (positive control) was administered ranitidine 10 mg/kgBW, groups 3, 4, and 5 were given n-hexane fraction, groups 6, 7 and 8 were given a water fraction, and groups of 9, 10, and 11 were given ethyl fractions with each group receives dose of 20, 40, and 80 mg/kgBW and all groups were treated for 3 days. Rats were dissected on 5th day for examination of gastric mucosal lesion size and performed ELISA expression of TNF-α expression of gastric mucosal tissue. The results of this study were assayed by SPSS 18.
Results: The result of the research using Kruskal-Wallis test showed that there were significant differences (p <0.05) of the lesions size between the sample groups where control positive, ethyl fraction 20, 40, 80 mg/kgBW, and water fraction 20, 40 mg/kgBB had the gastric mucosal lesion size differed significantly with the negative control group, while the TNF-α protein expression test using Kruskal-Wallis showed that there was a significant difference (p <0.05) TNF-α levels of all groups against the negative control.
Conclusion: Active gambir fraction had a potention to reduce size of mucose gaster lesion and reduce expression of TNF-α protein.
Authors
- Ni Wayan Lisa Suasti1*
- Lunsi Oktafitria1
- Ellya Aidia1
- Rahmad Dhani1
- 1Bachelor of Pharmacy Study Program, Sekolah Tinggi Ilmu Farmasi Bhakti Pertiwi, Palembang, Indonesia
Corresponding author Ni Wayan Lisa Suasti — dr.wayanlisa@gmail.com
Article history
- Submitted
- Accepted
- Published
Downloads
Published
Issue
Section
License
Copyright and licensing
Copyright in each article remains with the author(s). Authors grant Bioscientia Medicina: Journal of Biomedicine and Translational Research a non-exclusive right of first publication and the right to identify itself as the original publisher. No exclusive transfer of copyright is required.
All articles are published under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA 4.0): https://creativecommons.org/licenses/by-nc-sa/4.0/. Users may copy, redistribute, remix, transform, and build upon the material for non-commercial purposes, provided appropriate attribution is given, a link to the license is supplied, changes are indicated, and adaptations are distributed under the same license.
The license applies to the article's scholarly content unless a credit line states otherwise. Third-party material may be subject to separate rights. Authors retain patent, trademark, moral, and research-data rights. The copyright year follows the article's publication date.
