The Effect of Ethanolic Extract of Moringa oleifera Leaves on the Macrophage Count and VEGF Expression on Wistar Rats with Burn Wound
DOI:
https://doi.org/10.37275/bsm.v6i6.535Keywords:
Moringa oleifera, Silver sulfadiazine, Burn wound, Macrophage, VEGFAbstract
Background: One modality type of burn wound therapy is topical silver sulfadiazine. Recently, research about topical herb medicine has grown. Moringa oleifera (MO) is a kind of herb plant that has an anti-inflammation effect and has an influence on angiogenesis, so it tends to faster burn wound healing. This study aimed to prove the effect of ethanolic extract of Moringa oleifera leaves on the macrophage count and VEGF expression in Wistar rats with burn wounds.
Methods: The research design that was used is "Randomized post-test with a control group." The study population used 24 male Wistar rats that were induced burn wounds and randomly divided into 4 groups which were given medicine topically once daily for 7 days. Treatment groups included: I (MO leaves extract 10%), II (SSD + MO leaves extract 10%), III (SSD), dan IV (pure vehiculum). Exudation was assessed macroscopically, while PMN and macrophage amount were assessed microscopically with Hematoxylin-Eosin. Data were analyzed and processed using hypothesis test One Way ANOVA – Post Hoc Bonferroni, Kruskal Wallis – Mann Whitney, and correlation test Spearman and Pearson with SPSS 25.0 program.
Results: Significant difference obtained in macrophage amount between II group and IV group (p 0,000). There was no significant difference in VEGF expression. There was also no correlation between macrophage amount and VEGF expression.
Conclusion: Ethanolic extract of Moringa oleifera leaves extract proved to be effective in decreasing macrophage cell count on Wistar rats with burn wounds.
Authors
- Agnes Stella Valentina1*
- Najatullah2
- Trilaksana Nugroho3
- Neni Susilaningsih3
- 1General Surgery Resident, Faculty of Medicine, Universitas Diponegoro/ Dr. Kariadi General Hospital, Semarang, Indonesia
- 2Plastic Surgery Lecturer, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
- 3Biomedic Lecturer, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
Corresponding author Agnes Stella Valentina — agnes.stella.21@gmail.com
Article history
- Submitted
- Accepted
- Published
Downloads
Published
Issue
Section
License
As our aim is to disseminate original research article, hence the publishing right is a necessary one. The publishing right is needed in order to reach the agreement between the author and publisher. As the journal is fully open access, the authors will sign an exclusive license agreement.
The authors have the right to:
- Share their article in the same ways permitted to third parties under the relevant user license.
- Retain copyright, patent, trademark and other intellectual property rights including research data.
- Proper attribution and credit for the published work.
For the open access article, the publisher is granted to the following right.
- The non-exclusive right to publish the article and grant right to others.
- For the published article, the publisher applied for the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
