Potential of Sriwijaya Thermal Cycler Smart Controlling-Based as a Tool for DNA Sequence Polymerase Chain Reaction
DOI:
https://doi.org/10.32539/bsm.v5i4.208Keywords:
COVID-19, Control Groups, Severe Acute Respiratory Syndrome Corona virus 2, Polymerase Chain Reaction, DNAAbstract
Introduction. Along with the COVID-19 pandemic, the need for a thermal cycler
device for examining COVID-19 is getting bigger. Many laboratories have overused
the use of thermal cyclers due to the limited availability of this tool. This study aims
to test the effectiveness of the Sriwijaya thermal cycler based on a smart controller
in conducting PCR DNA sequences. Methods. The research design was an
experimental study with a posttest control group approach, in order to see an
overview of the results of the polymerase chain reaction (PCR) in the form of
intermediate DNA bands using Sriwijaya Thermal Cycler compared to groups using
factory-made Thermal cyclers. Results. The PCR results showed precise results from
the image and band separation, and there was a clear separation of the bands on
the marker. The PCR results from the ACE I / D gene showed quite good and optimal
results in the separation of the PCR results band. Conclusion. Sriwijaya thermal
cycler is effective in DNA polymerase chain reaction (PCR) sequences comparable to
that of the manufacturer's Thermal Cycler.
Authors
- Msy Rulan Adnindya1*
- Indri Seta Septadina1
- Muhammad Reagan 2
- 1Department of Anatomy, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
- 2Department of Internal Medicine, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
Corresponding author Msy Rulan Adnindya — msy.rulan@gmail.com
Article history
- Submitted
- Accepted
- Published
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