Accreditation
SINTA 2Nationally Accredited Journal
Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Articles
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The Role of Vascular Endothelial Growth Factorin Thrombocytosis in Colorectal Cancer Patients The Role of VEGF in Thrombocytosis
Views: 1,482Downloads: 726Backgorund: Colorectal cancer was included in a group of cancer with various complications. One complication that was often a cause of morbidity and mortality was thrombocytosis. In colorectal cancer, the incidence of thrombocytosis associated with the formation of blood vessels around the tumor or angiogenesis. Factors that played an important role in angiogenesis was vascular endothelial growth factor (VEGF).
Methods: This study was an observational analytic research in colorectal cancer patients to determine the correlation levels of platelets and serum VEGF levels. A total of 33 patients with colorectal cancer at the Palembang Mohammad Hoesin Hospital be research subjects to examine the levels of platelets and levels of VEGF. The level of serum VEGF was performed using ELISA technique from SIGMA®.
Results: The average level of the patient's platelets was281,090.9±105,860.8/mm3. In this study, two patients (6.06%) have thrombocytosis.The average serum levels of VEGF research subjects were 221.2 ± 152.8 pg/mL.Correlation test of levels of serum VEGF and platelets levels showed the value of p=0.040 (p> 0.05) and r = 0468.
Conclusions: Thus, it can be concluded that in this research serum VEGF levels are almost always causes an increase in platelet levels in patients with colorectal cancer.
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Hematologic profiles of Plasmodium vivax Malaria patients
Views: 1,577Downloads: 1,235Abstract
Background: Malaria infections cause various symptoms ranging from asymptomatic infections to severe disease complications. Plasmodium vivax malaria has been recognized as a disease that attacks blood cells, causing various hematologic changes, especially anemia, leukopenia, leukocytosis, neutropenia, neutrophilia, and thrombocytopenia with different percentages. Plasmodium vivax, formerly known to cause mild malaria, was later proven to cause severe malaria, even cerebral malaria such as Plasmodium falciparum. This study aims to determine the hematologic profile in patients with Plasmodium vivax malaria.
Method: This research use descriptive cross sectional design. This research was conducted in Puskesmas (PKM; Primary Health Care) Sukamaju and Puskesmas (PKM; Primary Health Care) Karang City in August until December 2017. Samples were taken by consecutive sampling. A total of 37 samples expressed positive Plasmodium vivax, examined their hematologic profiles specifically hemoglobin, erythrocytes, leucocytes, platelets, lymphocytes, neutrophils, monocytes and hematocrit using automatic hematology cell counter.
Results: Based on the results of this study, 56.76% of patients had anemia, 45.90% of patients had leukopenia, 89.20% thrombocytopenia, 2.70% neutrophilia, 10.80% neutropenia, 2.70% lymphocytosis, 35.10% lymphopenia, and 13.50% pancytopenia.
Conclusion: In patients with Plasmodium vivax malaria infection there may be a change in hematologic profiles, this change may be affected by the acute phase of infection and host immune system.
Keywords: Plasmodium vivax malaria, hematologic profiles
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Granulocyte Colony Stimulating Factor (G-CSF) Quantitative Analysis In Plasmodium Vivax Infected Malaria Patients Experiencing Trombocytopenia
Views: 1,443Downloads: 813AbstractIntroduction : P. Vivax has been refered as pathological factor underlying increasing prevalences of haematological abnormality including anemia and thrombocytopenia. Through the day, exact mechanism of thrombocytopenia in malaria infection has yet come to a conclusion, several hypothesis are still in considered, phagocytosis and platelets aggregation remain the major disscussion topics. G-CSF, cytocine with elevated serum quantity in P. Vivax infections, were responsible in increasing phagoctytosis and conducting direct effect on platelets aggregation using adequate ADP. Increasing number of ADP in malaria cases were correlated with erythrocyte haemolitic, leading to increasing platelets aggregation. Although numerous hypothesis has been compelled, only a-few research publication has been made corresponding to G-CSF serum level on malaria P. Vivax infection and its correlations to thrombocytopenic events.
Aim of study : To analyze the relations between G-CSF serum levels and parasite numbers towards platelets profile in infected malaria P. Vivax patients.
Methods : Study design using Prospective analysis. Thirty six patients with single infection of Malaria Vivax in Puskesmas Sukamaju (Primary Heath Care Centre) and Puskesmas Kota Karang were assessed for G-CSF plasma levels, platelet counts, and MPV. Data analysis were conducted using Spearman correlation methods with SPSS.
Results : Study results showing significant correlation between G-CSF serum levels towards Platelet conts (R = -0,397(p = 0,016)), without significant correlation between G-CSF and MPV value (p = 0,874)
Conclusion : G-CSF serum levels were related with thrombocytopenia, with no correlation towards MPV.
Keyword: G-CSF, P. vivax, Thrombocytopenia, MPV.
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The Efficacy of Seluang Fish Oil (Rasbora agrotynea) Related Body Weight, Lipid Profile, Adiponectin and Leptin in Wistar Rats-Induced High Fat Diet
Views: 2,725Downloads: 768Background
Rasbora agrotynea (local name : Seluang ) is a fish native to Sumatera, Malaya and Borneo. This fish has a potential as a local wisdom for supplementation of omega 3 and omega 6. In Sumatera, Seluang fish was used as traditional food.
Aim of Study
Aim of this study to explore the efficacy of seluang fish oil in body weight, lipid profile, adiponectin and leptin level in Wistar Rats-Induced High Fat Diet
Methods
This study was an experimental study , pre-post test with control group design. The sample in this study was 30 male rats, 8 weeks old, weight 150-200 gram. Rats were given high fat diet and seluang fish oil at dose of 0,1 , 0,2 and 0,4 mL/200 gr BW/day for 2 weeks. Seluang fish oil was extracted by distilation methods. The results of this study were assayed by SPSS 18.
Results
Seluang fish oil 0,4 mL/200 gr BW was more potent to reduce body weight gain, triglyseride level, leptin and increase adiponectin level than seluang fish oil 0,1 mL/200 gr BW, 0,2 mL/200 gr BW, negative control and positive control.
Conclusion
Seluang fish oil had a potention to reduce body weight, triglyceride, leptin and increase adiponectin level.
Keywords: Seluang fish Oil – body weight- triglyserida – leptin - adiponectin
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The Efficacy of Red Ginger Fraction (Zingiber officinale Roscoe var. rubrum) as Insecticidal Aedes aegypti
Views: 3,059Downloads: 3,146Aedes aegypti is a vector of Dengue Hemoragic Fever. The use of synthetic chemical insecticide is the most common way to control Ae. aegypti. However, since it can cause resistency if it is used in a wide scale with high frequency and continously, it requires an alternative way. Red ginger (Zingiber officinale Roscoe var. rubrum) is one of natural insecticide which is safe to be used to control Ae. aegypti. This research aims was to determine the activity of active fraction of red ginger to mortality of Ae. aegypti. It was a quasi-experiment research with post test only control design. Twenty Ae. aegypti Liverpool strain were the sample of this research with 5 concentration for each and 4 times repetition. The result showed that concentration 0.5%, 1.0%, 1.5%, 2.0% and 2.5% causing mortality percentage of 32.5%, 33.8%, 51.3%, 58.8% and 65.0% respectively. One way Anova test showed p-value < alpha ± (0.00 < 0.05). It was concluded that there was a significant difference of various concentration of active fraction n-heksan of red ginger extract to the mortality of Ae. aegypti. The lethal concentration (LC50) was 2.409%. It was suggested to continue this research with reppellant or bio-assay test method.
Key words: Toxicity, extract, fraction, Aedes aegypti, Insecticidal
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The Relationship Between Soil Transmitted Helminthes (STH) Infection and Nutritional Status in Students of State Elementary School Number (SDN) 200 Palembang Indonesia
Views: 1,850Downloads: 1,779Infection of Soil Transmitted Helminthes (STH) is caused by intestinal nematodes where as in its life cycle, soil is needed as the media for the eggs or larvae to mature into effective forms, most commonly happen to children of school age. This infection is still one of the main problems in public health, including Indonesia. STH infection are widely distributed in tropical and subtropical areas. Lack of personal hygiene, poor environmental sanitation and low socioeconomic status are some factors that plays role in increasing the occurrence of the infection. This infection is also one of the causes responsible for malnutrition in children by decreasing appetite and food intake thus ensued adverse consequences such as declining growth pace, impairment of physical health, and weakening cognitive function. This study was conducted to analyze the association of STH infection with nutritional status of SDN 200 students in Kertapati District, Palembang. This study was an analytic observational research with a cross sectional research design. Samples consist of 107 students chosen using proportional stratified random sampling technique. Data was collected by direct interview using questionnaires, measuring body weight and height to obtain nutritional status which then classified using CDC 2000 growth curve while fecal contamination was examined using Kato Katz and modified Harada Mori methode in the Laboratory of Parasitology Medical Faculty of Universitas Sriwijaya. Data then analyzed using Chi-square test. From 107 students, 27.1% infection of STH was found on 29 students with 6 students (20.7%) infected by A. lumbricoides and 23 students (79.3%) infected by T. trichiura. Proportion of malnutrition status was found at 43.9%. Statistical test showed a significant association between STH infection and nutritional status (p=0.036; OR=3.167; Cl 95%: 1.163-15.237). There was a significant association between STH infection and nutritional status in students of SDN 200 Kelurahan Kemas Rindo Kertapati District Palembang City.
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Efficacy of SEBCLAIRA® Cream for Moderate Seborrhoeic Dermatitis on The Face as An Adjuvant Therapy (Randomised Clinical Trial, Double Blind)
Views: 1,580Downloads: 2,229Background: Seborrhoeic dermatitis (SD) is a chronic papulosquamous inflammatory disease which resistant to medical treatment. Various treatment such as topical corticosteroid, antifungal and calcineurin inhibitor has been widely practiced and gives varying results
Objective: Our objective was to compare the efficacy of Sebclaira® and topical hydrocortisone 2,5% for management of moderate SD on the face
Methods: A randomised clinical trial, controlled, double blind study was performed for four weeks. We assessed the efficacy and side effects of these topical treatment. The severity of SD was evaluated using Seborrhea Area Severity Index-Facial (SASI-F) score. The severity of pruritus was evaluated using Visual Analogue Scale. Demographic characteristics, baseline SASI-F and VAS were recorded in the medical record.
Results: A 34 patients (14 males, 20 females) with moderate SD on the face completed the four weeks study. The mean of SASI T2 and VAS score of the Sebclaira® group was significantly lower than Hydrocortisone 2.5% group with p = 0.000 and p = 0.000 respectively. Tolerance between Sebclaira® and Hydrocortisone 2.5% showed insignificant results (p = 1.000)
Conclusions: The longer application of Sebclaira® was significantly more effective to improve moderate SD
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Risk Factors for Stunting Children Aged 6-59 Months In Pulau Panggung District, South Sumatera, Indonesia
Views: 2,954Downloads: 833Background :. Stunting among children is one of the major health problems in developing countries. Globally, 45% of deaths among children are caused by malnutrition, meanwhile stunting, as one of the factors, plays an important role. Children who experience stunting can not achieve optimal growth and cognitive potential. Hence, that will have an impact on future productivity. Muara Enim district is included in one district with stunting prevalence, which is above 20%, i.e. 26.9%. This study was to identify the risk factors of stunting children in Pulau Panggung district, Muara Enim, South Sumatera.
Methods: This study applied observational analytic research using cross sectional design. It was conducted on August to October 2017 at public health center in Pulau Panggung district, Muara Enim. There were 83 children who fullfilled the inclusion criteria. The relationship between risk factors and stunting was analyzed using Chi square test and Logistic Regression test was applied to identify the risk factor that play the most role to stunting.
Results: 49,4% out of 83 children suffer from stunting, with a mean age of 29,37±13,69 months where the number of children stunting with male gender is 53.7% while female gender is 46.3%. Chi square analysis showed that there was a significant relationship among birth weight, birth length, mother's occupation, history of infection, exclussive breastfeeding and stunting (p< 0.05) but no significant relationship was found among sex, mother's education, maternal height and income with stunting (p> 0.05). Multivariate Logistic Regression test showed that mother's occupation and history of infection are the risk factors that contibute the most to stunting among children aged 6-59 months in Pulau Panggung district. The risk of stunting among working mothers is higher 5 times than unworking mothers and children with infection history have risk of stunting 3 times compared to children without infection history.
Conclusion: Mother's occupation and infection history are risk factors that contribute the most to stunting among children at Pulau Panggung district, Muara Enim, South Sumatera.
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Association of Knowledge, Attitude and Practice of Cleaning Service with the Amount of Mosquito Larvae in Water Container At Bhayangkara Hospital, Palembang, Indonesia
Views: 1,383Downloads: 819Bacgkround: Efforts in the eradication of mosquito breeding have been done, but have not shown maximum results due to lack of community participation caused by several factors, such as lack of knowledge, attitude and practice that are considered to play important role in reducing the incidence of vector-borne diseases transmitted by mosquitoes. This study aimed was to determine the relationship of knowledge, attitude and practice of cleaning services with the amount of mosquito larvae at water container around Bhayangkara Hospital Palembang. Populations in this study were 25 cleaning services in Bhayangkara Hospital Palembang.
Methods: Samples in this study were all populations taken by using total sampling technique. Data included the number and types of water containers, the number and species of mosquito larvae found in the water containers, and data of knowledge, attitude and practice of cleaning services. The results were analysed using Kruskal-Wallis statistic test.
Results: There were 75 water containers, 63 water containers were indoors, one of them contains larvae, and 12 water containers were outdoors, the type of two outdoors water containers that contained larvae were plastic buckets. Total larvae that found were 16 larvae of Aedes aegypti. Based on Kruskal-Wallis statistic test, there was no average difference between the number of mosquito larvae based on knowledge with p>alpha± (0.072>0.05), and there was an average difference between the number of mosquito larvae based on attitudewith p<alpha± (0.006>0.05) and practice with p<alpha± (0.019<0.05).
Conclusion: There was a negative association between attitude and practice against the number of mosquito larvae, but not with knowledge.
