Accreditation
SINTA 2Nationally Accredited Journal
Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Articles
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Identification of Plasmodium Falciparum Merozoit Surface Protein-1 (PFMSP-1) Gene From Malaria Patients in Hanura Area, Lampung, Indonesia
Views: 1,399Downloads: 855Introduction: Malaria is a major health problem not only in Indonesia, but also the world. Incidence of anti malarial drug resistance has been found, and one of the possible cause is genetic factors. The changes that occur in the Plasmodium falciparum gene cause genetic variations that lead to resistance for treatment. There is a gene with high polymorphism that can be used as a gene marker of genetic variation in Plasmodium falciparum.
Aim of Study: This study aim to identification of plasmodium falciparum merozoit surface protein-1 (PFMSP-1) gene from malaria patients in Puskesmas Hanura, Pesawaran, Lampung.
Methods: Descriptive method with morbidity survey approach is used in this study. There are total 23 biological material stored that had been taken in 2016 from malaria patient in Puskesmas Hanura, Pesawaran, Lampung, by consecutive sampling. Identification of genetic variance of the Plasmodium falciparum gene was performed by nested PCR at Medical Faculty Biomolecular Laboratory of Lampung University. The results of this study were processed using a computer software.
Results: There are 23 samples that amplified following nested PCR for Plasmodium falciparum Merozoit Surface Protein-1 (PFMSP-1) gene target. In all samples studied, there are PFMSP-1 gene with six variations base pair length range from 200 to 2000 bp.
Conclusions: this study has successfully amplified PFMSP-1 gene from individual’s blood sample infected by Malaria in Puskesmas Hanura work region, Pesawaran, Lampung.
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The Comparison of High Flow Nasal Cannula Oxygen and Conventional Oxygen Therapy on Early Effect Hemodynamic Indicators in Post – Upper Abdominal Surgery Patients A Pilot Study
Views: 1,347Downloads: 815Background
The effect of high-flow nasal cannula (HFNC) on hemodynamic data of the patients is still unknown. This was a pilot study to evaluate the effect of HFNC and conventional oxygen therapy (COT) on post-upper abdominal surgery.
Methods
This was an open-label randomized controlled trial (RCT) at tertiary hospital between February-June 2019. Inclusion criteria were ASA III, aged 18-65 years, BMI 18,5-29 kg/m2. Exclusion criteria were patients with unstable hemodynamic with vasopressors, general oedema, and uncooperative. Thirty patients were recruited and divided into HFNC group (n=15) and COT group (n=15). Hemodynamic parameters were recorded using the bedside monitor (heart rate, respiratory rate, and mean arterial pressure) as well as the electrical cardiometry using ICON® measurements (stroke volume index, cardiac index and systemic vascular resistance index); laboratory parameters were ScVO2 and lactate serum. Data were collected at 0, 30 and 60 minutes post-extubation.
Results
The General Linear Model (GLM) tests on clinical parameters (HR, RR and MAP) were P= 0.20; P=0.72; P = 0.18, ICON® parameters (SVI, CI and SVRI) were P= 0.02;P =0.64; P =0.64 meanwhile ScVO2 and lactate were P = 0.35 and P = 0.22. Despite those results, at some period of measurements there were a tendency of a better outcomes of most parameters for the HFNC group.
Conclusion
According to the results, the only statistically significant outcome was SVI (P =0.02) after the analysis between those two groups. However, the HFNC group showed a clinically improvement of the other outcomes among post-upper abdominal surgery patients.
Keywords
High flow nasal cannula, conventional oxygen therapy, haemodynamic, abdominal surgery
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The Effect of Intravenous Vitamin C on Urine Neutrophil Gelatinase-Associated Lipocalin Among Septic Patients A Randomised Control Trial
Views: 1,100Downloads: 789Background
The role of vitamin C to decrease organ dysfunction in sepsis was still controversial. This study aimed to explore the effect of intravenous (IV) vitamin C on urine NGAL (uNGAL) levels among septic patients in ICU.
Methods
This study was a randomized clinical trial held in Cipto Mangunkusumo Hospital from April to July 2019 with consecutive sampling method. Patients aged > 18 years with sepsis based on the criteria of sepsis-3 who were admitted to the ICU were included in this study. Exclusion criteria were those with chronic kidney problems, with kidney stones, undergo renal replacement therapy in the ICU. All subjects were divided into: Group A was treated with combination of vitamin C and thiamine while Group B was given thiamine only. The uNGAL level was measured at baseline, 24, 48 and 72 h after treatment. Anova for repeated measurement using General Linear Model for Repeated Measurement was used with level of significant at p-value <0.05.
Results
Total of 33 subjects were included. In Group A we found uNGAL (ng/ mL) were 74.5 (13.3-102.9), 77.3 (15.2-98.4), 67.2 (22.6-100.6), 77.2 (17.0-100.5) for baseline, 24 h, 48 h, and 72 h respectively. While in Group B uNGAL were 57.7 (11.5-94.5), 57.1 (6.4-97.7), 53.7 (13.3-99.6), 47.6 (4.5-100.9). No significant difference in terms of uNGAL between two groups at each hour was found.
Conclusions
This study showed that intravenous vitamin C administration had no effect on uNGAL among septic patients. Need more study to investigate approaches to improve kidney and inflammatory biomarker among septic patients.
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Efficacy of cinnamon (Cinnamomum burmannii) extract to decrease serum creatinine in acute kidney injury induced male wistar rats
Views: 22,429Downloads: 1,067Background
Kidney vital function as a regulator of blood volume and chemical composition to excrete solute and water selectively. Acute kidney injury (AKI) is a sudden decline in kidney function which is temporary, is marked by an increase in serum creatinine levels and decreased urine output.
Objective
This study aims to determine the effectiveness of cinnamon extract in acute kidney injury induced male wistar rats.
Method
An experimental in vivo with pre-post control group design was conducted in twenty-five wistar strain white rats that were divided into 5 treatment groups that received methylprednisolone as a positive control, aquades, and different dose of cinnamon extracts (50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW). The rat model of acute kidney injury was prepared by the method of unilateral ureteral obstruction (UUO).
The effectiveness of cinnamon extract was carried out by creatinine levels checked using ELISA and analyzed by ANOVA.
Results
The extract of cinnamon can lower serum creatinine levels were significantly (p <0.05). A dose of 100mg / KgBW is an effective dose in AKI compare to methylprednisolone.
Conclusion
Extract of cinnamon (Cinnamomum burmannii) corrected creatinine levels of acute kidney injury induced male wistar rats.
Keywords: Acute kidney injury, cinnamon extract, creatinine
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The Anticancer Activity of Srikaya Leaves Fraction (Annona squamosa L.): An In Vitro Study
Views: 3,336Downloads: 986Abstract
Introduction: Anticancer drugs are aimed primarily at inhibiting the growth and proliferation of cancer cells. Srikaya leaves (Annona squamosa L.) had been proven to possess various therapeutic effects and potential to be developed as anticancer drugs due to its cytotoxic activity.
Aim of study: This study aimed to assess the anticancer activity of srikaya leaves (Annona squamosa L.) fraction.
Methods: Methanol fraction of srikaya leaves were obtained at concentrations of 500; 250; 125; 62.5; 31.25 µg/ml. Srikaya methanol fraction and cisplatin as control were given to a plate that was sealed with T47D cells for MTT assay. Identification of compounds in the methanol fraction of srikaya leaves was performed with thin layer chromatography (TLC). Data were collected in the form of absorbance value and half-maximal inhibitory concentration (IC50) value was determined ​​by linear regression. Data analysis was carried out with paired T test, unpaired T test, and ANOVA.
Results: Average percentage of T47D cells viability increased with the decrease in the concentration of srikaya methanol fraction. Obtained IC50 value was 174.25 µg/ml which was quite active and potential to be developed as an anticancer drug. Methanol fraction of srikaya leaves contained secondary terpenoid metabolites, steroids, phenols, flavonoids, alkaloids and tannins. Flavonoid was the dominant metabolites in phytochemical tests and believed to play a major role in cytotoxic activity of srikaya leaves.
Conclusion: Methanol fraction of srikaya leaves possessed the cytotoxic effect on T47D cancer cell line through the role of flavonoid metabolites.
Keywords: srikaya, Annona squamosa, anticancer, T47D cells
