Accreditation
SINTA 2Nationally Accredited Journal
Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Articles
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Human Papilloma Virus: Biomolecular Aspect
Views: 1,280Downloads: 939HPV is a sexually transmitted virus, and high-risk HPV DNA was found in 99.7%
of cervical cancer specimens. Within 12 to 24 months of exposure to the virus,
90% of HPV infections disappear or become inactive. However, infection with high-
risk strains of HPV persist which then increases the risk of progression to cervical
cancer. The detection of precancerous lesions consists of various methods,
including pap smear (conventional or liquid-base cytology / LBC), visual
inspection of acetic acid (IVA), visual inspection of lugoliodine (VILI), and HPV DNA
test (genotyping / hybrid capture). -
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model
Views: 1,285Downloads: 553Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14.
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Laboratory Examination of Syphilis
Views: 2,223Downloads: 4,888Syphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal disease research laboratory (VDRL), toluidine red unheated serum test (TRUST), unheated serum reagin (USR), rapid plasma reagin (RPR) and treponemal serologic test, such as T. pallidum passive particle agglutination (TPPA), T. pallidum haemagglutination assay (TPHA), fluorescent treponemal antibody absorption (FTA-Abs), enzyme immunoassay (EIA) and rapid test. The algorithm of serologic test can be divided into traditional or reverse.
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Risk Factors for Congenital Deafness in Pediatric Patients Who Underwent Otoaccoustic Emission (OAE) and Auditory Brainstem Response (ABR) Examinations in General Hospital Mohammad Hoesin Palembang, Indonesia
Views: 1,838Downloads: 737Introduction: Congenital deafness is a hearing loss that occurs at birth. Congenital deafness in neonates can be caused by risk factors during pregnancy and during the birth process. The tests carried out for hearing screening for neonates in hospital up to 1 month old are Otoaccoustic Emission (OAE) and Auditory Brainstem Response (ABR) examinations.
Objective: Determining the relationship between family history of deafness, syndromes associated with sensorineural hearing loss, TORCH infection and prenatal syphilis, use of ototoxic drugs during pregnancy, prematurity, low birth weight, asphyxia, and hyperbilirubinemia with the incidence of congenital deafness in children
Methods: This cross-sectional study was conducted based on medical record datas from children who underwent OAE and ABR examinations at Dr. Mohammad Hoesin Palembang hospital from January 2019 to February 2021.
Results: From the 349 children, 180 (51.6%) had bilateral OAE and ABR pass results, 161 (46.1%) had bilateral referrals and 8 (2.3%) children received unilateral refer results. From 122 children with risk factors, 38 (31.1%) children with bilateral passes, 81 (66.4%) children with bilateral referrals and 3 (2.5 %) children with unilateral refer. From 227 children without risk factors, 142 (62.6 %) children with a bilateral pass, 80 (35.2 %) children with bilateral referrals and 5 (2.2%) children with unilateral refer. The most common risk factor was LBW of 41 (11.7%) children. Chi square test and logistic regression analysis results showed a significant relationship between ototoxic drugs during pregnancy and congenital deafness (p = 0.001) with referral results, the value of Odd Ratio (OR) 9.651.
Conclusions: There is a significant relationship between risk factors for ototoxic drugs during pregnancy, TORCH and syphilis infection during pregnancy, asphyxia, congenital syndrome, LBWand hyperbilirubinemia with congenital deafness.
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Genital Herpes in Human Immunodeficiency Virus Infected Patients
Views: 1,559Downloads: 5,172Genital herpes is a recurrent, lifelong sexual transmitted infection caused by HSV, especially type 2. Genital herpes is the most common infection in HIV patient. HSV-2 can increase the risk of HIV acquisition 2 to 3 times. Clinical manifestations of genital herpes can be different between HIV- infected and non-HIV patients. HIV-infected patients have a high risk of developing chronic and severe genital ulcers with atypical manifestation, prolonged healing, and resistant to treatment, depends on CD4 count. Genital herpes can be diagnosed based on history, clinical manifestation, laboratory and histopathologic examination. Management of genital herpes includes education and counseling patients and sexual partners, systemic antiviral, and symptomatic treatment.
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Fluid and Drug Administration Procedure Animal Model in Biomedical Research
Views: 1,832Downloads: 2,602Drugs, vaccines, injectable anesthetics or other agents are to be administered, one or more of several different routes may be selected. The routes selected are governed by the nature of the agent being administered, the animal, the purpose of the administration and other factors.
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Clinical Efficacy of the Use of Honey as Wound Treatment in Surgical Site Infection due to Hysterectomy Patient with Type 2 Diabetes Mellitus
Views: 2,241Downloads: 1,121Introduction: Honey is believed to be used in the topical treatment of wounds and burns due to its anti-bacterial and wound healing promotion activities. The sugar content in honey is high enough to inhibit microbial growth. This case report reports the clinical efficacy of using honey as a postoperative hysterectomy wound dressing with type 2 diabetes mellitus complications.
Case Presentation: A woman, 40 years old, lived in Palembang, housewife, came with complaints of open surgical scars and smelling pus. The patient is a postoperative patient with cesarean section with indications of a prolonged latent phase and macrosomia. The patient also had a history of uncontrolled diabetes mellitus since 8 years ago. Intraoperatively, a wound dehiscence is obtained in the lower uterine segment and adhesions between the anterior uterine body and the omentum and transverse colon. The patient finally underwent a supracervical hysterectomy and the surgical secretions were examined for bacterial culture and antibiotic resistance. During wound care, the patient's surgical wound was treated using honey dressings. Patient experienced improvement after treatment for 12 days post-operation.
Conclusion: Honey has antibacterial and tissue regeneration ability which is high enough to heal surgical wounds. Further studies are needed for the application of honey to postoperative wounds more widely
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Efficacy of Aloe Vera Hydrogel in Inhibition The Growth of Campylobacter rectus and Provetella intermedia Bacteria
Views: 1,320Downloads: 1,090Introduction: Campylobacter rectus and Provetella intermedia bacteria as the main etiology causes of periodontal disease. One of the locally synthesized antimicrobials that is widely used for the treatment of periodontitis is Chlorhexidine, however this drug has side effects when used continuously. To overcome this, an alternative periodontitis therapy with medicinal plants is needed. This study aims to determine the effectiveness of hydrogel Aloe vera antibacterial concentrations of 2.5%, 5%, 10% and 20% in the bacteria Campylobacter rectus and Provetella intermedia.
Methods: This study was a laboratory experimental study with posttest only control group design in vitro. The samples were Campylobacter rectus and Provetella intermedia. The number of groups in this study was six. The sample size was determined by Federer's formula and obtained four replications for each group. Data collection by measuring the inhibition diameter using a slide caliper. Data were analyzed using oneway ANOVA and posthoc LSD.
Results: The results showed that there was a difference in the diameter of the hydrogel Aloe vera inhibition with a concentration of 2.5%, 5%, 10%, 20% and a positive control for Campylobacter rectus and Provetella intermedia bacteria.
Conclusion: This study concluded that there is an antibacterial effectiveness of hydrogel Aloe vera in concentrations of 2.5%, 5%, 10% and 20% on Campylobacter rectus and Provetella intermedia bacteria.
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The Incidence Rate of Cranii Base Fracture in Dr. Kariadi Hospital Semarang Period 2019
Views: 1,133Downloads: 545Introduction: Head injury is a major cause of death, especially in young adults and a major cause of disability. The incidence of head injuries is increasing due to the increasing use of motorized vehicles. As many as 4% of cases of head fracture occur in the base of the cranii.
Method: This research used a descriptive observational method. The data used is secondary data taken from medical records. In this study, we wanted to know the incidence of cranii base fracture cases due to head trauma in patients treated at Dr. Kariadi period 2019.
Result: From 29 samples of medical records obtained, 48.3% had mild head trauma, 27.6% had moderate head trauma, and 24.1% had severe head trauma. Symptoms of patients with the most clinical symptoms of cranii base fracture were raccoon eyes 51.7%, with the most fracture locations in the anterior fossa as much as 51.7%.
Conclusion: The incidence of cranii fracture due to head trauma at RSUP Dr. Kariadi in 2019 was found mostly over 40 years old and most of them were men. The type of injury is a minor head injury, with the largest location in the anterior fossa, and the most common clinical sign found in patients with cranii fracture is raccoon eyes.
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Jugular Vein Aneurysm, When We Have to Do Surgery?
Views: 1,430Downloads: 1,830Introduction: The most common lesions of jugular vein dilatation are aneurysms and ectasia. A jugular vein aneurysm is less common compare to an arterial aneurysm in adults. Because of the rare incidence, treatment guidelines primarily associated with the timing of surgery are not clearly established. Proper treatment can reduce patient complaints without excessive intervention.
Case report: A 54 years old woman complained of swelling in the right neck that started three years ago and cephalgia for two years. From CT angiography, we obtained a jugular vein dilatation of 2.3 cm. During periodic evaluation from ultrasonography doppler, there is no increase in the size of the jugular vein.
Conclusion: Jugular vein aneurysm presenting in adults is an infrequent phenomenon. It is a benign condition, and conservative observation is advised. It should be operated only if symptomatic or progressive enlarging. A periodic examination must be done to evaluate the size of the jugular vein before a surgical decision
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Improving the Quality of Life for the Elderly with Degenerative Diseases through to the Charitas Group Virtual Health Education Program during the Pandemic of the COVID-19
Views: 1,053Downloads: 862Background: In the era of the COVID-19 pandemic, various problems were experienced by the elderly, especially those with degenerative diseases, as the population most at risk of being exposed to COVID-19. The elderly are vulnerable to decreased physical, mental and social health; therefore, a new and effective strategy is needed to improve healthy living behavior in this population. This study aimed to analyze the effect of the Charitas Group virtual health education program on improving the quality of life of the elderly with degenerative diseases in the era of the COVID-19 pandemic.
Methods: The One Group Pretest-Posttest Pre-Experimental study was with a sample of 33 patients selected by purposive sampling in May - July 2021. The data collection was taken using the World Health Organization Quality of Life – Bref (WHOQOL-Bref) form, translated and validated into Bahasa Indonesia. The data was then compared before and after the intervention and analyzed using the Paired Samples T-Test.
Results: The T-Test showed a significant difference between the pre-test and post-test results (p-value 0.041 < 0.05). At the same time, the other T-Test results showed a significant influence on the quality of life of the elderly in the specific domains, namely: Psychological (p-value 0.025), Social Relationship (p-value 0.033) and Environmental (p-value 0.034).
Conclusions: The Charitas Group virtual health education program significantly influences the quality of life of elderly people with degenerative diseases in the COVID-19 pandemic era.
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Factors Related To Outcome of Traumatic Brain Injury Patients at M Djamil Padang Hospital
Views: 864Downloads: 459Background: Traumatic brain injury is still a major threat because it can cause global morbidity and mortality. Many factors can affect the outcome of a traumatic brain injury. Some conditions that can exacerbate traumatic brain injury include GCS conditions, blood pressure variability, and pupillary reflexes.
Methods: The research was conducted in M. Djamil Padang Hospital from October 2020 to March 2021. The study design was a cross-sectional study in traumatic brain injury patients with ≤ 48 hours of onset and the aged between 18-60 years. The subjects in this study consisted of 66 subjects. At 6 weeks after onset, a GOS assessment was performed to assess patient outcomes. Statistical analysis was performed computerized with SPSS 22.0. P-value <0.05 was considered statistically significant.
Results: Most of the patients were male (71.2%) with an average age of 36.41 ± 14,275 years, and the most common injury mechanism was traffic accidents (95.5%). There was a significant relationship between onset of incidence, hypotension, pupillary reflexes, and Rotterdam score with the outcome of traumatic brain injury patients (p<0.05) and there was no significant relationship between age, gender, and mechanism of injury with the outcome patients with traumatic brain injury.
Conclution: The onset of events, hypotension, pupillary reflexes, and Rotterdam scores significantly affect the outcome patients of traumatic brain injury.
