Personalized Periodontal Regeneration: Integrating 3D Bioprinting with Autologous Activated Growth Factor (AGF) for Enhanced Alveolar Bone Defect Healing
DOI:
https://doi.org/10.37275/bsm.v9i12.1605Keywords:
3D bioprinting, Alveolar bone defect, Autologous activated growth factor, Periodontal regeneration, Tissue engineeringAbstract
Background: The complete structural restoration of alveolar bone defects remains a critical challenge in reconstructive periodontology. Conventional grafting methods often fail to replicate the complex microarchitecture of periodontal tissues. This study integrated three-dimensional bioprinting technology with Autologous Activated Growth Factor to construct personalized scaffolds for treating severe infrabony periodontal defects.
Methods: A randomized controlled clinical study was conducted at a private hospital in Palembang, South Sumatera, involving patients with Stage III periodontitis exhibiting vertical alveolar bone defects. Cone-beam computed tomography data guided the precise three-dimensional bioprinting of polycaprolactone and beta-tricalcium phosphate scaffolds. These constructs were functionalized operatively with Autologous Activated Growth Factor prepared from peripheral blood. The control group received conventional deproteinized bovine bone mineral with a collagen membrane. Clinical attachment level, probing depth, and radiographic bone fill were evaluated over six months.
Results: The three-dimensional bioprinted scaffolds functionalized with Autologous Activated Growth Factor demonstrated superior clinical outcomes. At six months, the experimental group showed a mean probing depth reduction of 4.82 mm and a clinical attachment level gain of 4.15 mm. Radiographic analysis revealed a mean bone fill of 82.4% in the experimental group compared to 64.1% in the control. Inflammatory biomarkers were significantly modulated, indicating a highly favorable regenerative microenvironment.
Conclusion: The synergistic integration of personalized three-dimensional bioprinted scaffolds with the potent osteoinductive and angiogenic properties of Autologous Activated Growth Factor significantly enhanced alveolar bone defect healing, providing a highly predictable alternative for complex periodontal regeneration.
Authors
- Zelka Dapala1*
- Rachmat Hidayat2
- Muhammad Ashraf3
- 1Department of Dentistry, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
- 2Department of Medical Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
- 3Department of Oral Health and Dentistry, Phlox Institute, Palembang, Indonesia
Corresponding author Zelka Dapala — azelkadapala@gmail.com
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