Accreditation
SINTA 2Nationally Accredited Journal
Vol. 5 No. 7 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Articles
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Protocol for Anesthesia Animal Model in Biomedical Study
Views: 1,394Downloads: 470Recognition of pain depends upon intact pathways from pain receptors to the thalamus and cerebral cortex, as well as functional cerebral cortex and subcortical structures. Thus any means that renders the cerebral cortex nonfunctional, such as hypoxia or drug depression, prevents pain. When this happens, stimuli that evoke motor nerve reflexes that may be painful to the conscious animal are not painful in the unconscious animal. Equally painful stimuli administered to animals chemically paralyzed by curare or succinylcholine will not evoke a motor reflex simply because of paralysis, but will cause pain because of the conscious state. Hence, it is possible that unconscious animals may feel no pain but respond to certain stimuli, and paralyzed animals may feel pain but cannot respond.
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Epidemiology and Risk Factors for Cervical Cancer
Views: 5,522Downloads: 6,051Cervical cancer in Indonesia in 2018 ranks second in cancer in women in Indonesia with an incidence rate of 348.809 cases with a mortality rate of nearly 60% of the incidence, namely 207.210 deaths. Deaths from cervical cancer are projected to continue to increase and are estimated to reach 12 million deaths by 2030 if not treated properly. The incidence of cervical cancer in Indonesia is estimated to have 180.000 new cases per year and the death rate is thought to reach 75% in the first year. This death is mainly associated with the majority of newly diagnosed patients who are already at an advanced stage (70% of cases) and are already at the terminal stage at the time of diagnosis.
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Granuloma Inguinale
Views: 1,901Downloads: 3,767Granuloma ingunale (GI) or donovanosis is a genital ulcer disease caused by the
Calymmatobacterium granulomatis. It is a Gram-negative, facultative, obligate
intracellular and pleomorphic bacterium. This bacterium has phylogenetically
closed to and placed within the Klebsiella genus. Clinically, the disease is com-
monly characterized as painless, slowly progressive ulcerative lesions on the
genitals or perineum without regional lymphadenopathy. The lesions are highly
vascular and bleed easily on contact Extragenital lesions may occur but are rare
and more common in newborns from mothers with GI genital lesions. This
disease is often neglected, therefore it is often misdiagnosed and inaccurate
therapy. Treatment time is 3 weeks or until clinical cure has been achieved for
all proposed regimens. It often occurs both in men and women of reproductive
age (20-40 years). This article consists of several theoretical references that have
been viewed to have a better understanding of GI. -
The Ethanolic Extract of Annonaceous sp. to Inhibit Vascular Endothelial Growth Factor (VEGF) as Anticancer Modalities
Views: 1,138Downloads: 477Introduction: Cancer has become a major public health problem worldwide, with breast cancer is the most common cancer among women and one of the most important causes of death among them. Management of breast cancer have some adverse effects, with many factorx=s shiouln be considered to ordered the therapy. Use of chemotherapy will suppress cell proliferation and trigger apoptosis, but it cannot just focus on cancer cells only, but also would affected on other cells and cause.
Objective: This study aim to analyze the inhibition effect of the Annonanceous sp. ethanolic extract to Vascular Endothelial Growth Factor (VEGF) on MCF-7 cells line
Material and Methods: This study is an analytic experimental study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured used RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. were exposed to MCF-7 cells for 48 hours. Analyze of VEGF level use ELISA Methods with λ=405 nm.
Results: The results of ELISA analyze shows that the ethanolic extract of Annonaceous sp. have potential effect to decreased of VEGF expression on sample with treatment on some concentration (p< 0,05).
Conclusion: The ethanolic extract of Annonaceous sp. has shows the potential effect to decreased of VEGF level on MCF-7 cells line
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Comparison of Germ Patterns and Antimicrobial Susceptibility in Chronic Suppurative Otitis Media with Cholesteatoma and without Cholesteatoma in Dr. Mohammad Hoesin Hospital.
Views: 1,370Downloads: 749Introduction: Chronic suppurative otitis media (CSOM) is a chronic inflammation of the middle ear and mastoid cavity. It is necessary to identify bacterial patterns and antibiotics susceptibility in CSOM. Appropriate administration of antibiotics is essential to treat infection and prevent widespread resistance.
Objective: The aim of this study is to compare the pattern of germs and antibiotic susceptibility between CSOM with cholesteatoma and without cholesteatoma.
Methods: Observational study with cross-sectional design. Data collection was carried out using medical record data on 102 subjects who underwent middle ear swab and bacterial culture from January to December 2019.
Results: From 102 subjects and 122 bacterial culture results. it was found that Pseudomonas aeruginosa (36.1%) was one of the most common organisms to cause CSOM. followed by Staphylococcus haemolyticus. Staphylococcus epidermidis and Staphylococcus aureus. There was a significant difference between the germ pattern and the type of CSOM (p = 0.002). Amikacin. meropenem. gentamicin. cefepime. ciprofloxacin and ceftazidime were found to be the most effective antibiotics for Pseudomonas aeruginosa. The rate of resistance to tigecycline and cefazoline were highest. Age. sex. nutritional status and comorbidities were not factors that significantly influenced the incidence of cholesteatoma in CSOM.
Conclusion: There is a significant difference between the germ pattern in both CSOM groups. Patients that show Gram negative have a significant effect on the incidence of CSOM with cholesteatoma.
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The Effect of Hydrogel Aloe vera (Aloe vera (L.) Burm) on the Number of Neutrophil Cells in Aggressive Periodontitis Induced by Aggregatibacter actinomycetemcomitans (In Vivo Study on Wistar Rats)
Views: 1,455Downloads: 849Introduction: Aggressive periodontitis is a periodontal disease that runs rapidly, characterized by gross alveolar bone loss, the disease is unprompted by complaints, not related to local aspects.
Objective: The aim of this study was to determine the effect of Aloe vera hydrogel on the number of neutrophil cells in aggressive periodontitis induced by A. actinomycetemcomitans in Wistar rats.
Methods: This type of research is a laboratory experimental design with post-test only design with control group design. This study used 24 Wistar rats divided into 6 groups, namely, negative control group (Basic hydrogel), ibuprofen and hydrogel Aloe vera 2.5%, 5%, 10%, 20%.
Results: The test results for the mean number of neutrophil cells in the hydrogel aloe vera group of 2.5%, 5%, 10%, 20%, ibuprofen, and negative controls were 120.50 ± 12.92, 78.25 ± 7.37, 47, 25 ± 4.03, 1.00 ± 0.82, 0.50 ± 0.58, and 217.25 ± 62.26. The results of the oneway Anova statistical test obtained p value = 0.001, which means that there is a difference in the number of neutrophils of Wistar rats in all treatment groups.
Conclusion: The conclusion of this study there is the effect of giving hydrogel aloe vera a concentration of 2.5%, 5%, 10%, 20% with ibuprofen on Wistar rats induced by aggressive periodontitis.
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Lung Abscess Carcinomatous
Views: 1,166Downloads: 2,071Introduction: Pulmonary malignancies may easily be overlooked and valuable time may be lost. Lung cancer is sometimes diagnosed as a tuberculous cavity or an abscess. Abscess formation can appear in several different ways. Carcinoma that occurs in medium-sized bronchi causes partial bronchial obstruction, atelectasis, and infection due to retention. Inflammation can progress to damage to lung tissue, resulting the formation of multiple suppurative foci or more localized lung abscess. The link between lung abscess and lung cancer has been known, but the presence of malignancy in lung abscesses often undiagnosed. Obstruction from lung cancer can predispose to the development of a lung abscess. Case of a 54 year old man with increased pain at the right chest when breathing in since two months. On physical examination, it was found decreased of fremitus at the right hemithorax, deafness and decreased breath sounds as high as the II - V thoracic right hemithorax. Thorax CT-scan showed a round, homogeneous (HU: 17-30), with a cavity-like image with air fluid level, size: 7,88 cm x 8,2 cm x 9,29 cm and honey comb appearance around it. On the examination of TTNA (Transthoracic Needle Aspiration) results obtained Squamous Cell Carcinoma. A lobectomy is planned for the patient.
Conclusion: We reported a rare case, a 54 year old male patient, with the diagnosis of Carcinomatous Lung Abscess. This case report was prepared with the aim of increasing awareness of malignancy in patients with a clinical presentation of abscesses, especially in old age.
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Covid-19 in Lupus Nephritis
Views: 1,352Downloads: 580COVID-19 outbreak is currently being concerned for managing patients with
immunological disorders nowadays, including SLE. Lupus is a complex
autoimmune disease characterized by the presence of autoantibodies that against
cell nucleus involved many organs in the body. Patients with SLE will increase
risk of severe infection because the intrinsic respond attack with their immune
respond though immunosuppressive drugs consumption, and will potentially
damage some organs target associated with their disease. Lupus have multiple
clinical manifestations with a fluctuating symptom. Patient who come with the
symptom ofbreathlessness will getworse day by day. The symptom could be felt in
the same time as fatigue, joint pain, hair loss, malar rash, oral ulcer, pleural
effusion and swollen feet. There's a patient with antinuclear antibody positive for
anti-smith and anti-Ro/SS-A. She was diagnosed with COVID-19, SLE with
nephritis, haemolytic anemia, vasculitis and pleural effusions. The clinical
manifestations of this patient indicate a COVID-19 with lupus nephritis that has
severe disease. She was being treated with methylprednisolone and
hydroxychloroquine for SLE and azithromycin plus oseltamivir as a therapy for
COVID-19. The effect of hydroxychloroquine on SARS-CoV-2 was better seen in
patients with SLE who gotthe medication regularly. Patients went home after 24
days of hospitalization after negative RT-PCR results and clinical improvement of
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The Effectivity of Supplementation Artemisia vulgaris for Adenocarcinoma Mammae Chemotherapy to Reduce CD 34 and Tumor Massa Diameter (Study in C3H Mice Given Adriamycin - Cyclophosphamide Chemotherapy)
Views: 1,140Downloads: 545Introduction: Breast cancer is still a major health problem in the world. In the case of breast cancer, surgery is the main treatment option besides chemotherapy, radiation, and immunotherapy such as Artemisia vulgaris (AV). AV is cytotoxic selectively acts as a supplement to breast adenocarcinoma chemotherapy given the Adriamycin-Cyclophosphamide regimen, to improve chemotherapy response.The study was aimed to proving AV extract enhances the chemotherapy response in C3H mice with adenocarcinoma mammae given Adriamycin-Cyclophosphamide Chemotherapy.
Method: This study used Post test only control group design on 24 females C3H mice that were randomly selected and divided into four groups: group K (control), P1 (chemotherapy), P2 (extract), and P3 (combination). Adenocarcinoma mammae comes from the inoculation of donor mice. Chemotherapy of Adriamycin 60 mg / m 2 and Cyclophosphamide 600 mg / m 2 were given in two cycles. AV 13 mg (0.2 ml) was given once daily orally. CD34 were evaluated by imunohistochemical staining and tumor mass diameter were counted by calipers.
Result: The microvascular density CD34 and tumor mass diameter were obtained in groups of K, P1, P2, P3 respectively 60.76 ± 1.5; 39.70 ± 2.00; 57.10 ± 1.29; 35.26 ± 2.06 and 12.52 ± 1.49; 6.20 + 1.04; 9,94 + 1.21; 3.94 + 0.76. Statistical analysis showed significant differences in CD34 between groups K vs P1, P2, P3 (p = 0.001, p = 0.014, p = 0.001), P1 vs P2 and P3 (p = 0.001, p = 0.033) and P2 (P = 0.001). Tumor mass diameter between groups K vs P1, P2, P3 (p=0.001; p=0.014; p=0,001), P1 with P2 (p= 0.001) P1 with P3 (p = 0.033) and P2 with P3 (p = 0.001). Correlation analysis between CD34 with tumor mass diameter was found to have significant correlation (p = 0.001 and r = 0.932).
Conclusion: Artemisia vulgaris is a potential to reduce angiogenesis in terms of decreasing the microvascular density CD34 and tumor mass diameter of adenocarcinoma mammae of C3H mice treated with Adriamycin-Cyclophosphamide chemotherapy and can improve the effectivity.
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COVID-19 Health Protocol and Religious Activities: Knowledge, Attitude, and Compliance among Generation Z
Views: 1,375Downloads: 634Background: COVID-19 (coronavirus disease 2019) is a contagious infection currently affecting people worldwide, including in Indonesia. The spread of this virus is extremely rapid, and the number of deaths continues to rise. Compliance with the health protocol is one method of preventing the spread of COVID-19. The purpose of this study is to determine the relationship between generation-Z (gen-Z) knowledge and attitudes toward the use of masks, keeping distance, and washing hands as a means of preventing COVID-19 in religious activities at houses of worship in Palembang.
Method: The observational analytic cross-sectional research design with the study's population consists of generation Z members who participate in religious activities in places of worship. The cluster random sampling method yielded 147 respondents. The data collected are primary data obtained directly from the research subjects via a questionnaire and secondary data obtained from the Palembang City Ministry of Religion. They were using the Pearson chi-square test to analyze descriptive data.
Results: According to the findings, 79 people (53.7%) out of 147 respondents have good knowledge, while 75 people have a good attitude (51% ). The results obtained a statistically significant p-value of 0.05, indicating a relationship between gene Z knowledge and attitude and adherence to the use of masks, keeping distance, and washing hands.
Conclusion: There is a significant relationship between knowledge and attitudes toward COVID-19 infection prevention in Generation Z who participate in religious activities in Palembang City.
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Zinc Serum Maternal Levels as a Risk Factor for Preeclampsia
Views: 1,272Downloads: 1,006Background. Preeclampsia is defined as gestational hypertension and proteinuria which can be measured by the elevation of liver transaminases to twice normal concentration, and the symptoms of increased intracranial pressure such as dizziness, blurred vision, and seizures. Zinc is an essential mineral for many biological functions and one of the trace elements that is directly involved in the oxidative/antioxidant balance, a pathogenesis process in preeclampsia that is highly dependent on dietary habits and supplements. This study was aimed to assess the association between serum zinc level with preeclampsia (PE).
Methods: This observational, case-control study was performed on 90 women with singleton pregnancies at 28 to 42 weeks of gestation which were referred to the Department of Obstetrics and Gynecology, Dr. Mohammad Hoesin General Hospital, Palembang, between August 2020 to November 2020. 30 pregnant women with PE were selected as cases and 60 healthy pregnant women were selected as controls. Maternal serum zinc samples were collected. The collected data were entered into the master table. Tabulation, coding, and statistical data analysis were performed using SPSS version 20.0 for Windows.
Results: The mean serum zinc levels in the case group and control group were 43,90 ± 15,79 µg/dL (ranged from 15 to 86 µg/dL) and 48,77 ± 10,54 µg/dL (ranged from 22 to 83 µg/dL), respectively. A value of serum zinc levels with the best sensitivity and specificity was 45,5 µg/dL.
Conclusion: There was a significant association between maternal serum zinc level with PE. The risk of PE was 3,2 times higher in pregnant women with serum zinc levels of ≤45,5 mcg/dL compared to pregnant women with serum zinc levels of >45,5 mcg/dL. In this case it is necessary to supplement zinc earlier in pregnancy to reduce the risk of preeclampsia.
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Sensitivity and Specificity of Vascular Endothelial Growth Factor A (VEGF-A) against Endometriosis
Views: 903Downloads: 435Background. Endometriosis is a benign disorder defined by the presence of endometrial glands and stroma outside the uterus. Endometriosis occurs in 10-15% of women during their reproductive years. Angiogenesis and the inflammatory response are important factors in the development of endometriosis. The formation of a new blood supply is a crucial step in the formation of endometrial lesions. Angiogenesis is induced by a growth factor peptide, namely vascular endothelial growth factor A (VEGF-A). VEGF-A is known as a vascular permeability factor that plays an important role in the pathological angiogenesis process and is a more specific and prominent angiogenesis factor among the VEGF family. The purpose of this study was to determine the sensitivity and specificity of examination VEGF-A of menstrual blood in diagnosing endometriosis compared to laparoscopy. Methods. This diagnostic test research has been carried out at the gynecology polyclinic, especially the division of Fertility, Endocrinology and Reproduction, Faculty of Medicine, Sriwijaya University- Dr. Central General Hospital. Mohammad Hoesin from August to November 2018, there were 45 patients who met the inclusion criteria. VEGF-A examination in instrumental blood based on ELISA examination. Data analysis to measure sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy using Medcalc statistics. Results. From the results of this study, it is known that the majority of the study samples were aged 31.69 years (24-38 years) 75.6%, body mass index (BMI) 51.1% with normal BMI, experienced infertility by 82.2% of the sample, most of the samples did not smoke (95.6%) and most experienced mild pain 55.6% using the VAS pain scale. From this study, it is known that 84.4% or 38 of the study samples had endometriosis with a cut off point value of VEGF-A > 347 pg/mL. From the results of this study, it is known that the sensitivity of VEGF-A in diagnosing endometriosis is 84.2%, specificity is 85.7%, positive predictive value is 97%, negative predictive value is 50% and accuracy is 84%. Conclusion. VEGF-A menstrual blood can be used as a diagnostic tool for endometriosis.
