Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein
DOI:
https://doi.org/10.37275/bsm.v5i4.229Keywords:
Western blotting, Protein, AntibodyAbstract
Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target proteins using appropriate primary and secondary antibodies to visualize. This paper will attempt to explain the techniques and theory behind western blot, and offer several ways to solve the problem
Authors
- Rachmat Hidayat1*
- Patricia Wulandari2
- 1Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
- 2Cattleya Mental Health Center, Palembang, Indonesia
Corresponding author Rachmat Hidayat — dr.rachmat.hidayat@gmail.com
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- Submitted
- Accepted
- Published
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